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New therapy extends breast cancer survival rate, prevents reoccurrence – Medical Xpress

A new immunotherapy developed by researchers at Northwestern University dramatically extends the survival time of mice with triple negative breast cancer, one of the most aggressive and difficult-to-treat forms of breast cancer.

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A new immunotherapy developed by researchers at Northwestern University dramatically extends the survival time of mice with triple negative breast cancer, one of the most aggressive and difficult-to-treat forms of breast cancer.
In a new study, mice treated with the therapy, which comprises two immunity-boosting drugs housed inside a nanoparticle, experienced complete tumor remission for at least 100 days. All untreated mice died by day 30. None of the treated mice experienced adverse side effects or autoimmune responses.
The nanoparticle, called a spherical nucleic acid (SNA), is a globular form of DNA that can easily enter and stimulate immune cells. Northwestern’s Chad A. Mirkin, who led the study and invented SNAs, credits the nanoparticle’s shape and structure for the immunotherapy’s success.
“We have demonstrated that the overall structural presentation of a cancer vaccine or immunotherapeuticnot simply the active chemical componentscan dramatically impact its potency,” Mirkin said. “This finding is opening doors in an emerging field we call ‘rational vaccinology’ and could lead to treatments for many different types of cancer.”
The research will be published online during the week of July 13, 2020 in the Proceedings of the National Academy of Sciences.
Mirkin is the George B. Rathmann Professor of Chemistry in Northwestern’s Weinberg College of Arts and Sciences, director of the International Institute of Nanotechnology and member of the Robert H. Lurie Comprehensive Cancer Center of Northwestern University.
How it works
Typical immunotherapies consist of a molecule or molecules from tumor cells (called antigens) paired with a molecule (called an adjuvant) that stimulates the immune system. More advanced forms consist of a cocktail of antigen molecules taken from a patient’s cancer cells (called lysates). The lysate trains the immune system to recognize its target (the tumor), and the adjuvant boosts the body’s immune response to destroy that target. Physicians mix the lysate and adjuvant together in a cellular culture and then inject the mixture into the patient.
Because the therapy is a structurally ill-defined mixture, Mirkin calls this the “blender approach.” The lysate and adjuvant aren’t packaged together, so it’s difficult to ensure that they will hit the same target.
“Statistically, you will get some cells to take up both the lysate and the adjuvant,” said Cassandra Callmann, a postdoctoral fellow in Mirkin’s lab and the paper’s first author. “But you also will get some cells that only receive one or the other. To maximize the immunotherapy’s potency, you need to co-deliver both to the same target cells and in the most effective form or structure possible.”
To overcome this challenge, Mirkin’s team packaged the lysate and adjuvant together inside the core of an SNA. In the study, they injected the SNA underneath the skin of mice with triple-negative breast cancer. The SNAs traveled to the lymph nodes of the mice, entered the cells and released their cargo. This caused an immune response within the cells to combat the lysate.
Prolonging survival, preventing reoccurrence
After treating nine mice with triple-negative breast cancer, six experienced complete tumor remission for 100 days without obvious side effects. Although the other three mice never reached remission, the new treatment did suppress their tumor growth, and the mice still lived longer than the control group.
“It’s definitely prolonging survival,” Callmann noted. “Even if not all mice were completely cured.”
Mirkin and his team also found that the SNA-based immunotherapy protected the mice from relapsing. After the mice entered remission, the team attempted to reimplant the mice with cancer, but tumors did not grow.
When Mirkin’s team removed and examined the tumors from therapy-treated mice, the researchers found an increased number of cytotoxic T-cellsa type of immune cell that attacks diseaseand a decreased number of immunosuppressant cells, which prevent the immune system from responding to fight disease.
“If the immunotherapy is protecting the mice from cancer reoccurring, then we may be able to use this is in a preventive context,” said Mirkin, who is a member of the Robert H. Lurie Comprehensive Cancer Center of Northwestern University. “Our study suggests that the therapy is providing an ‘immune memory.’ That’s something we’re looking into right now.”
Interestingly, a stronger immune response occurred when the researchers incorporated oxidized tumor cells into the SNAs. When making the lysate, the researchers treated the tumor cells with hypochlorous acid, which oxidizesand killsthe cells. Other researchers have noted in previous clinical studies that oxidized cells create more potent immunotherapies.
“We have confirmed that’s true,” Callmann said. “And we showed that the immune system gives an even better response if the oxidized lysate is also packaged in an SNA.”
Exploring other cancers
Mirkin’s team first tested the new therapy on triple-negative breast cancer tumors because the cancer is one of the most difficult to treat. According to the Triple Negative Breast Cancer Foundation, this disease accounts for 15%-20% of all breast cancers. The cancer tests negative for three proteins (hence the name “triple negative”) produced in large amounts by other types of breast cancer. It resists commonly used breast cancer drugs that target those three proteins.
“It’s one of the most deadly and aggressive forms of breast cancer,” Callmann said. “There are many different types of mutations, and some of the cells mutate very quickly. There is an immediate need for new treatments that work.”
The researchers believe that, in theory, SNA-based immunotherapies should be an effective treatment for many types of cancer. Mirkin’s team plans to explore that next. Mirkin notes that he feels encouraged that four SNA drugs are already in human clinical trials, including a variant of the SNA used in this study in a type of immunotherapy for Merkel Cell carcinoma. That structure also was invented at Northwestern and is in a Phase 2 clinical trial conducted by Exicure, a clinical stage biotechnology startup.
More information:
Cassandra E. Callmann el al., “Tumor cell lysate-loaded immunostimulatory spherical nucleic acids as therapeutics for triple-negative breast cancer,” PNAS (2020). www.pnas.org/cgi/doi/10.1073/pnas.2005794117
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New therapy extends breast cancer survival rate, prevents reoccurrence (2020, July 13)
retrieved 13 July 2020
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The latest on the coronavirus pandemic: Live updates – cnn.com

The coronavirus pandemic has brought countries to a standstill. In many places, as countries reopen, Covid-19 cases are on the rise. Follow here for the latest.

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Experts are giving mixed messages about the Covid-19 risks of air travel as Americans consider their Thanksgiving holiday plans.  
A Harvard University study released Tuesday modeled the airflow in airliners and say the specialized onboard ventilation systems filter out 99% of airborne viruses. 
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Maine sees increase of more than 50 COVID-19 cases for 3rd consecutive day – WMTW Portland

The Maine Center for Disease Control and Prevention reported an increase of 57 coronavirus cases on Tuesday and no new deaths.

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AUGUSTA, Maine —The Maine Center for Disease Control and Prevention reported an increase of 57 coronavirus cases on Tuesday and no new deaths.
The number of Mainers with COVID-19 who have died remains at 146.
The 57 additional cases bring the total in Maine since the outbreak began to 6,311.
It was the third consecutive day Maine saw a net increase of more than 50 coronavirus cases.
Maine’s current 7-day average of 49.4 new daily cases is approaching the peak of 52.6 set in late May.
According…

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‘White matter lesion’ mapping tool can detect early signs of cognitive decline – News-Medical.Net

A new tool for analyzing tissue damage seen on MRI brain scans can detect with more than 70 percent accuracy early signs of cognitive decline, new research shows….

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Reviewed by Emily Henderson, B.Sc.Oct 27 2020
A new tool for analyzing tissue damage seen on MRI brain scans can detect with more than 70 percent accuracy early signs of cognitive decline, new research shows.
The findings by imaging specialists at NYU Grossman School of Medicine center on small bright spots on scans called white matter hyperintensities. Increased numbers and size of the intense-white spots seen on the mostly gray images of the brain have long been linked to memory loss and emotional…

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